Lipase Activity of Mucor pusillus1

Lipase of Mucor pusillus.

Lipase of Mucor pusillus NRRL 2543 was recovered with ammonium sulfate precipitation, gel filtration on Sephadex G-75, and anion-exchange chromatography on diethylaminoethyl-Sephadex A-50. Maximal glycerol ester hydrolase (lipase) activity was observed at pH 5.0 to 5.5 and 50 C when trioctanoin and olive oil were used as substrates. The enzyme also showed esterase activity; it hydrolyzed, with ...

Lipase Activity of Mycoplasma.

Enzymatic activity of lipase

The knowledge of the digestive system of bullfrogs is an important step for the determination of their nutritional requirements throughout growth phases. With the objective of evaluating the enzymatic activity of lipase in the intestinal content of bullfrogs (Rana catesbeiana Shaw, 1802), 100 animals with median weight of 3.6 g were distributed in stalls under controlled temperature and photope...

Determination of lipase activity.

Heretofore most methods for the determination of lipase activity have employed emulsions of fatty acid esters in water (1). These emulsions have been stabilized by use of such agents as gum acacia. The disadvantages of such methods are that (1) the emulsions break up to a considerable and variable extent during the course of the incubation, and (2) the enzyme is presumed to be in the aqueous ph...

University of Groningen Immobilization of Mucor miehei Lipase onto Macroporous Aminated Polyethersulfone Membrane for Enzymatic Reactions

Immobilization of enzymes is one of the most promising methods in enzyme performance enhancement, including stability, recovery, and reusability. However, investigation of suitable solid support in enzyme immobilization is still a scientific challenge. Polyethersulfone (PES) and aminated PES (PES–NH2) were successfully synthesized as novel materials for immobilization. Membranes with various po...